ISSN 1671-5411 CN 11-5329/R
Volume 20 Issue 5
May  2023
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Please cite this article as: XU R, WU J, YANG CJ, KANG L, JI YY, LI C, DING ZW, ZOU YZ. A circRNA–miRNA–mRNA network analysis underlying pathogenesis of human heart failure. J Geriatr Cardiol 2023; 20(5): 350−360. DOI: 10.26599/1671-5411.2023.05.004
Citation: Please cite this article as: XU R, WU J, YANG CJ, KANG L, JI YY, LI C, DING ZW, ZOU YZ. A circRNA–miRNA–mRNA network analysis underlying pathogenesis of human heart failure. J Geriatr Cardiol 2023; 20(5): 350−360. DOI: 10.26599/1671-5411.2023.05.004

A circRNA–miRNA–mRNA network analysis underlying pathogenesis of human heart failure

doi: 10.26599/1671-5411.2023.05.004
Funds:  This study was funded by the National Natural Science Foundation of China (81900245, 81730009, 81941002 and 81700256).
All data analyzed in this study came from the public articles[22-26].
*The authors contributed equally to this manuscript
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  •  BACKGROUND The molecular mechanisms of heart failure (HF) are still poorly understood. Circular RNA (circRNA) has been discovered in the heart in increasing numbers of studies. The goal of this research is to learn more about the potential roles of circRNAs in HF.  METHODS & RESULTS  We used RNA sequencing data to identify the characteristics of circRNAs expressed in the heart and discovered that the majority of circRNAs screened were less than 2000 nt. Additionally, chromosomes One and Y had the most and least number of circRNAs, respectively. After excluding duplicate host genes and intergenic circRNAs, a total of 238 differentially expressed circRNAs (DECs) and 203 host genes were discovered. However, only four of the 203 host genes of DECs were examined in HF differentially expressed genes. Another study used Gene Oncology analysis of DECs host genes to elucidate the underlying pathogenesis of HF, and it found that binding and catalytic activity accounted for a large portion of DECs. Immune system, metabolism, and signal transduction pathways were significantly enriched. Furthermore, 1052 potentially regulated miRNAs from the top 40 DECs were collected to build a circRNA-miRNA network, and it was discovered that 470 miRNAs can be regulated by multiple circRNAs, while others are regulated by a single circRNA. In addition, a comparison of the top 10 mRNAs in HF and their targeted miRNAs revealed that DDX3Y and UTY were regulated by the most and least circRNA, respectively.  CONCLUSION  These findings demonstrated circRNAs have species and tissue specific expression patterns; while circRNA expression is independent on host genes, the same types of genes in DECs and DEGs worked in HF. Our findings would contribute to a better understanding of the critical roles of circRNAs and lay the groundwork for future studies of HF molecular functions.
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