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中国人民解放军总医院
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中国人民解放军总医院老年心血管病研究所
中国科技出版传媒股份有限公司
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中华老年多器官疾病杂志编辑委员会
100853, 北京市复兴路28号
电话:010-66936756
传真:010-66936756
E-mail: zhlndqg@mode301.cn
创刊人 王士雯
总编辑 范利
副总编辑 陈韵岱
执行主编 叶大训
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
友情链接
吴克芬, 胡 予.D-半乳糖致衰老动物模型的建立及评价[J].中华老年多器官疾病杂志,2012,11(1):74~78
D-半乳糖致衰老动物模型的建立及评价
Establishment and evaluation of D-galactose induced aging animal model
  
DOI:10.3724/SP.J.1264.2012.00018
中文关键词:  D-半乳糖; 衰老; 模型, 动物
英文关键词:D-galactose; aging; models, animal
基金项目:
作者单位E-mail
吴克芬, 胡 予 复旦大学附属中山医院老年病科, 上海 200032 hu.yu@zs-hospital.sh.cn 
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中文摘要:
      建立衰老动物模型是研究人类衰老过程的有效方法。结合动物的生理特性及自然属性, 建立不同的动物模型, 已成为衰老及抗衰老研究的焦点。D-半乳糖衰老动物模型与其他几种衰老动物模型(如自然衰老模型、臭氧损伤衰老模型、去胸腺衰老模型、SAMP系小鼠衰老模型等)相比, 简便易行, 价格低廉, 结果稳定, 因而得到广泛应用。结合该模型近年来的运用和发展, 本文对该模型构建原理、剂量、具体方法和评估手段作一综述, 并对行为学水平、生化水平、形态学水平、分子生物水平检测指标进行总结。总之, 采用D-半乳糖 120~125mg/(kg?d)连续皮下注射6~8周是较为可靠、稳定的建立衰老动物模型的方法。
英文摘要:
      Establishment of aging animal model is an effective way to study the aging process of human being. It has been a focus to establish different aging models for research of aging and anti-aging agents according to the physiological characteristics and natural attributes of various animals. Compared with other aging models, including naturally aging model, ozone induced aging model, thymus removed aging model, and aging SAMP model, D-galactosis induced aging model has been widely used because of its easy feasibility, low lost, and stable performance. In this paper, regarding its application and development, we reviewed the D-galactosis induced aging models from the following aspects: the mechanism and the dosage of the D-galactose to induce aging, the specific protocols, and the evaluation for successful establishment. In addition, we summarized some indexes to assess aging, such as behavioral, biochemical, morphological and molecular biological indices. In summary, subcutaneous injection of 120~125mg/(kg?d) of D-galactose once per day for 6 to 8 weeks is a reliable and stable way to establish the aging model.
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