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中国人民解放军总医院老年心血管病研究所
中国科技出版传媒股份有限公司
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中华老年多器官疾病杂志编辑委员会
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E-mail: zhlndqg@mode301.cn
创刊人 王士雯
总编辑 范利
副总编辑 陈韵岱
执行主编 叶大训
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
友情链接
易军,李泱,朱庆磊,尹彤,杨洁,刘杰,赵晓静,周浩,陈光辉.葛根总黄酮上调磷脂酰肌醇3激酶/蛋白激酶B保护内皮细胞损伤[J].中华老年多器官疾病杂志,2017,16(12):939~943
葛根总黄酮上调磷脂酰肌醇3激酶/蛋白激酶B保护内皮细胞损伤
Flavonoids of puerarin protects vascular endothelial cells against hydrogen peroxide induced injury via up-regulating PI3K/AKT
投稿时间:2017-06-22  修订日期:2017-08-28
DOI:10.11915/j.issn.1671-5403.2017.12.218
中文关键词:  葛根总黄酮;内皮细胞损伤;凋亡;自噬
英文关键词:flavonoids of puerarin; endothelial cell injury; apoptosis; autophagy
基金项目:
作者单位E-mail
易军 解放军总医院心血管内科,北京 100853  
李泱 解放军总医院心血管内科,北京 100853 ganfang9876@163.com 
朱庆磊 解放军总医院心血管内科,北京 100853  
尹彤 解放军总医院心血管内科,北京 100853  
杨洁 解放军总医院心血管内科,北京 100853  
刘杰 解放军总医院心血管内科,北京 100853  
赵晓静 解放军总医院心血管内科,北京 100853  
周浩 解放军总医院心血管内科,北京 100853  
陈光辉 解放军总医院心血管内科,北京 100853 13910669498@163.com 
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中文摘要:
      目的 探讨葛根总黄酮(PR)对H2O2诱导的内皮细胞氧化损伤保护作用及其可能机制。方法 分离培养大鼠主动脉内皮细胞。MTT法检测细胞活力,免疫印迹检测PR对PI3K和AKT蛋白表达的影响,流式细胞仪检测细胞凋亡情况并进行细胞自噬荧光分析。组间比较采用单因素方差分析。结果 与PR+H2O2组相比,PR+H2O2+LY294002组的A570 nm值显著降低[(2.07±0.08) vs(1.69±0.04),P<0.05]。与对照组相比,H2O2组(25.47%)和PR+H2O2组(13.26%)的细胞凋亡率均显著增加(P<0.05);而与H2O2组相比,PR+H2O2组的细胞凋亡率亦显著降低(25.47% vs 13.26%;P<0.05)。与对照组相比,H2O2组和PR+H2O2组的自噬荧光强度均显著增高(P<0.05),PR+H2O2组的自噬荧光强度显著低于H2O2组(P<0.05)。H2O2+PR组的PI3K和AKT蛋白表达显著高于对照组(P<0.05),PR+H2O2+LY294002组的PI3K和AKT蛋白表达显著低于H2O2+PR组(P<0.05)。结论 PR可通过抑制细胞过度自噬和凋亡,促进内皮细胞存活,对H2O2诱导的内皮细胞损伤有保护作用,且其作用机制可能与调控PI3K/AKT表达上调相关。
英文摘要:
      Objective To investigate the protective effect of total flavonoids in puerarin (PR) on oxidant injury in endothelial cells (ECs) induced by hydrogen peroxide (H2O2) and the possible mechanism. Methods ECs were isolated from the aorta of male Sprague-Dawley rats by adherent method. Cell viability was assessed by MTT colorimetry, and the effect of PI3K inhibitor LY294002 on the proliferation was also measured. The cell apoptosis was detected with flow cytometry. Fluorescence microscopy was used to observe the autophagic marker, microtubule-associated protein 1 light chain 3 (LC-3) in the 3 groups of cells. Western blotting was employed to detect the expression of PI3K and AKT in presence or absence of LY294002. One-way ANOVA was used to make compar-ison between groups. Results The cell viability was significantly lower in the H2O2+PR ECs with LY294002 treatment than those without[(2.07±0.08) vs (1.69±0.04), P<0.05]. Flow cytometry showed that the apoptosis rate was significantly higher in the H2O2 injured cells and the injured cells with PR treatment than the control cells (P<0.05), but PR treatment reduced the apoptosis rate in the H2O2 cells (13.26% vs 25.47%, P<0.05). Compared with the control cells, the total intracellular LC3-GFP fluorescence intensity was enhanced in the H2O2 ECs and the H2O2+PR ECs (P<0.05), with that of the latter group more significant (P<0.05). The protein levels of PI3K and AKT were up-regulated significantly in H2O2+PR ECs than the control cells (P<0.05), while the increases were blocked by LY294002 treatment (P<0.05). Conclusion PR protects ECs from H2O2 induced injury and promotes cell survival through inhibiting cell excessive apoptosis and autophagy, which may be associated with up-regulation of PI3K/AKT expression.
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