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中国人民解放军总医院老年心血管病研究所
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中华老年多器官疾病杂志编辑委员会
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创刊人 王士雯
总编辑 范利
副总编辑 陈韵岱
执行主编 叶大训
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
友情链接
唐正和,康怀兰,刘翠明.沉默信息调节因子1对早期2型糖尿病大鼠脂联素的调节作用[J].中华老年多器官疾病杂志,2018,17(6):452~457
沉默信息调节因子1对早期2型糖尿病大鼠脂联素的调节作用
Regulatory effect of silent information regulator 1 on dynamic level of serum adiponectin in early stage of diabetic mellitus in rats
投稿时间:2018-01-08  修订日期:2018-03-19
DOI:10.11915/j.issn.1671-5403.2018.06.101
中文关键词:  糖尿病;肝损伤;沉默信息调节因子1;脂联素受体
英文关键词:diabetes mellitus; liver injury; silent informationregulator 1; adiponectin receptor
基金项目:
作者单位E-mail
唐正和 山东莱芜钢铁集团有限公司医院内分泌科,莱芜 271126 lgyytzhh@126.com 
康怀兰 山东莱芜钢铁集团有限公司医院内分泌科,莱芜 271126  
刘翠明 山东莱芜钢铁集团有限公司医院内分泌科,莱芜 271126  
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中文摘要:
      目的 检测脂联素水平及沉默信息调节因子1(SIRT1)在早期糖尿病(DM)大鼠肝脏组织中的表达变化,探究SIRT1对脂联素的调控作用。方法 Sprague-Dawley大鼠42只,分为4组:正常对照组(n=10)、DM组(n=10)、DM+白藜芦醇(RES)组(n=11)和DM+尼克酰胺(NIA)组(n=11)。高脂饮食联合链脲佐菌素建立2型DM大鼠模型。进行基础代谢和血清学检测,HE染色法观察肝脏病理结构变化,酶联免疫吸附试验(ELISA)试剂盒检测血清脂联素水平;Western blotting和逆转录-聚合酶链反应法检测肝组织SIRT1和脂联素受体(AdipoR)等表达变化。采用SPSS 19.0软件进行数据处理。组间比较采用方差分析及t检验。结果 与DM组相比,空腹血糖(FBG)在DM+RES组显著降低(P<0.05);总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)在DM+RES组显著降低(P<0.01),在DM+NIA组显著升高(P<0.05);甘油三酯(TG)在DM+RES组显著降低(P<0.05);高密度脂蛋白胆固醇(HDL-C)在DM+RES组显著升高(P<0.01)。与DM组相比较,DM+RES组的脂联素水平略有升高(P>0.05),DM+NIA组血清脂联素水平显著降低(P<0.05)。显微镜下,DM组大鼠肝脏可见不同程度炎细胞浸润、甚至变性,DM+RES组大鼠肝脏脂肪变性减轻,DM+NIA组大鼠肝脏肝细胞散在小泡性脂滴。与DM组相比较,DM+RES组AdipoR2和SIRT1蛋白表达显著增加(均P<0.05),WISP-1蛋白表达显著降低(P<0.05),而DM+NIA组AdipoR2和SIRT1蛋白表达显著降低。与DM组比较,AdipoR2和SIRT1 mRNA在DM+RES组表达显著增加(P<0.01),WISP-1 mRNA表达显著降低(P<0.01);而DM+NIA组AdipoR2表达显著降低(P<0.05)。结论 在早期DM肝损伤的发生发展过程中,SIRT1信号分子可能通过调节AdipoR的表达来调控脂联素的水平,参与DM内分泌系统的病理生理演变。
英文摘要:
      Objective To detect the level of adiponectin (APN) and liver expression of silent information regulator 1 (SIRT1) in rats in early stage of diabetic mellitus (DM) and investigate the role of SIRT1 in the regulation of APN. Methods A total of 42 Sprague-Dawley rats were randomly divided into normal control group (n=10), DM model group (n=10), DM+resveratrol (RES) group (n=11) and DM+niacin amide (NIA) group (n=11). Rat DM model was established by an injection of streptozocin after 5 weeks′ high fat diet. Basic metabolic indices, serological parameters, and pathological changes of liver tissues by HE staining were used to identify the model establishment. The plasma level of APN was detected by ELISA reagent kit. Western blotting and RT-PCR were employed to measure the expression of SIRT1 and APN receptor AdipoR2 at protein and mRNA levels in the liver tissues. SPSS statistics 19.0 was used to perform the statistical analysis. Chi-square test and Student′s t test were employed for intergroup comparison based on different data types. Results Compared with the DM group, the DM+RES group had significantly lower fasting blood glucose (FBG; P<0.05), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C; P<0.05), and triglycerides (TG; P<0.05), but obviously elevated high-density lipoprotein cholesterol (HDL-C; P<0.01), and slightly increased APN (P>0.05). The DM+NIA group had remarkable higher TC and LDL-C (P<0.05), and obviously decreased APN (P<0.05) when compared with the DM group. Pathological observation displayed that obvious infiltration of inflammatory cells and even degeneration in the liver tissues of the DM group, attenuated cell degeneration in the DM+RES group, and dispersed lipid droplets in the liver cells of the DM+NIA group. Western blotting showed the protein levels of AdipoR2 and SIRT1 were increased (both P<0.05) and that of WISP-1 was decreased in the DM+RES group (P<0.05), while the levels were reduced in the DM+NIA group. Similar trends were seen in the mRNA expression levels of the 2 molecules(P<0.05). Conclusion In the development of early liver damage in DM rats, the signaling molecules of SIRT1 may modulate the expression of AdipoR to regulate APN level, and then participate in the pathophysiological evolution of endocrine system after DM.
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