在线办公
期刊论坛
主 管
中国人民解放军总医院
主 办
中国人民解放军总医院老年心血管病研究所
中国科技出版传媒股份有限公司
编 辑
中华老年多器官疾病杂志编辑委员会
100853, 北京市复兴路28号
电话:010-66936756
传真:010-66936756
E-mail: zhlndqg@mode301.cn
创刊人 王士雯
总编辑 范利
副总编辑 陈韵岱
执行主编 叶大训
编辑部主任 王雪萍
ISSN 1671-5403
CN 11-4786
创刊时间 2002年
出版周期 月刊
邮发代号 82-408
友情链接
王德杰,郑傲.槲皮素对D-半乳糖致衰老小鼠模型学习记忆及脑内炎症的影响[J].中华老年多器官疾病杂志,2018,17(9):691~695
槲皮素对D-半乳糖致衰老小鼠模型学习记忆及脑内炎症的影响
Effects of quercetin on learning and memory and cerebral inflammation in the D-galactose-induced aging mice
投稿时间:2018-05-21  修订日期:2018-06-14
DOI:10.11915/j.issn.1671-5403.2018.09.158
中文关键词:  炎症;槲皮素;糖基化终末产物,高级;糖基化终末产物受体;D-半乳糖
英文关键词:inflammation; quercetin; glycation end products, advanced; receptor for glycation end products; D-galactose
基金项目:
作者单位E-mail
王德杰 山东电力中心医院企业健康管理中心,济南 250001 wdjmyj@163.com 
郑傲 山东电力中心医院企业健康管理中心,济南 250001  
摘要点击次数: 42
全文下载次数: 70
中文摘要:
      目的 探讨槲皮素对D-半乳糖致衰老小鼠模型学习记忆能力及脑内炎症通路的影响。方法 将昆明小鼠 32只随机分为对照组(NC组)、模型组(M组)、Q1治疗组(Q1组)、Q2治疗组(Q2组),每组8只。M组、Q1组及Q2组小鼠皮下注射D-半乳糖100 mg/(kg·d)建立小鼠衰老模型,NC组给予等量生理盐水。Q1、Q2组造模同时给予不同剂量槲皮素5、10 mg/(kg·d)灌胃干预,NC组和M组给予相同剂量生理盐水灌胃处理,干预8周。Morris水迷宫评价逃避潜伏期、穿越平台次数及平台象限滞留时间,Western blotting检测小鼠脑内高级糖基化终末产物(AGEs)及其受体(RAGE)、核转录因子(NF-κB)和其下游炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、环氧化酶-2(COX-2)表达水平。采用SPSS 20.0统计软件对数据进行分析。组间比较采用完全随机设计的单因素方差分析或t检验。结果 与NC组比较,M组小鼠逃避潜伏期延长、穿越平台次数及在平台所在象限滞留时间降低,脑内AGEs、RAGE、NF-κB及炎症因子TNF-α、IL-1β、COX-2水平显著升高(P<0.05)。与M组比较,治疗组小鼠逃避潜伏期缩短、穿越平台次数及在平台所在象限滞留时间延长,脑内炎性因子水平减少(P<0.05)。与Q1组比较,Q2组小鼠逃避潜伏期缩短、穿越平台次数及在平台所在象限滞留时间延长,RAGE、IL-1β和COX-2表达水平显著降低。结论 槲皮素能够有效改善衰老小鼠学习记忆功能障碍,抑制AGEs/RAGE/NF-κB炎症通路,降低脑内炎性因子的生成,具有抗衰老潜力。
英文摘要:
      Objective To investigate the effects of quercetin on learning and memory and pathway of cerebral inflammation in D-galactose-induced aging mice. Methods Thirty-two Kunming mice were randomly divided into control group (NC group), model group (M group), Q1 treatment group (Q1 group) and Q2 treatment group (Q2 group), with 8 in each group. The aging mouse model was induced by subcutaneous administration of D-galactose 100 mg/(kg·d) in M, Q1 and Q2 groups, and the same amount of normal saline was administered in the same way in the NC group. Quercetin were then given in different doses in Q1 [5 mg/(kg·d)] and Q2 [10 mg/(kg·d)] groups, and the same dose of saline was given to the NC group and M group. Intervention duration was 8 weeks. Morris water maze was used to assess the escape latency, frequency of crossing the platform and retention time in the platform quadrant in the mice. Western blotting was employed to detect the expression of advanced glycation end products (AGEs) and their receptor (RAGE), nuclear factor-κB (NF-κB) and its downstream inflammatory factors, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2). SPSS statistics 20.0 was used for data analysis, and one-way ANOVA for complete randomized design or Student′s t test for comparison between the groups. Results Compared with NC group, M group had lower escape latency, fewer crossings and shorter retention in target quadrant, but significantly higher AGEs, RAGE, NF-κB, TNF-α, IL-1β and COX-2. Compared with M group, treatment groups had lower escape latency, more crossings and longer retention in the quadrant of the platforms, and lower indices of cerebral inflammation (P<0.05). Compared with Q1 group, Q2 group had lower escape latency, more crossings and longer retention in the quadrant of platforms, and significantly lower expression of RAGE, IL-1β and COX-2. Conclusion Quercetin could effectively improve learning and memory dysfunction in the aging mice and reduce production of inflammatory factors in the brain by inhibiting AGEs/RAGE/NF-κB inflammatory pathway, hence having antiaging potential.
查看全文    下载PDF阅读器
关闭