A circRNA-miRNA-mRNA network identifcation for exploring underlying pathogenesis of human heart failure

Ran XU; Jian WU; Chun-Jie YANG; Le KANG; Yu-Yao JI; Chang LI; Zhi-Wen DING; Yun-Zeng ZOU

doi:10.11909/j.issn.1671-5411.2022.10.016

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Ran XU, Jian WU, Chun-Jie YANG, Le KANG, Yu-Yao JI, Chang LI, Zhi-Wen DING, Yun-Zeng ZOU. A circRNA-miRNA-mRNA network identifcation for exploring underlying pathogenesis of human heart failure. J Geriatr Cardiol. doi: 10.11909/j.issn.1671-5411.2022.10.016

Citation:

Ran XU, Jian WU, Chun-Jie YANG, Le KANG, Yu-Yao JI, Chang LI, Zhi-Wen DING, Yun-Zeng ZOU. A circRNA-miRNA-mRNA network identifcation for exploring underlying pathogenesis of human heart failure. J Geriatr Cardiol. doi: 10.11909/j.issn.1671-5411.2022.10.016

Citation:

Ran XU, Jian WU, Chun-Jie YANG, Le KANG, Yu-Yao JI, Chang LI, Zhi-Wen DING, Yun-Zeng ZOU. A circRNA-miRNA-mRNA network identifcation for exploring underlying pathogenesis of human heart failure. J Geriatr Cardiol. doi: 10.11909/j.issn.1671-5411.2022.10.016

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A circRNA-miRNA-mRNA network identifcation for exploring underlying pathogenesis of human heart failure

doi: 10.11909/j.issn.1671-5411.2022.10.016

1.
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China

Funds: This study was funded by the National Natural Science Foundation of China (No.: 81900245, 81730009, 81941002 and 81700256).

None
All data analyzed in this study came from the public articles^[22-26].

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Corresponding author: zou.yunzeng@zs-hospital.sh.cn (ZOU YZ)
Available Online: 2022-08-30

Abstract

Abstract

BACKGROUND The molecular mechanisms of heart failure (HF) remain poorly understood. Studies have increasingly found circular RNA (circRNA) exists in heart. The aim of this study is to acquire potential functions of circRNAs in HF. METHODS & RESULTS We integrated RNA sequencing data to identify the characteristics of circRNAs expressed in heart and found that most circRNAs screened were < 2000 nt (89%); moreover, Chromosome 1 and Y contained the largest and least number of circRNAs (10.59% and 0.2%), respectively. Subsequently, a total of 238 differentially expressed circRNAs (DECs) were found and 203 host genes were discovered after excluding duplicate host genes and intergenic circRNAs. However, only 4 of 203 host genes of DECs were checked in differentially expressed genes (DEGs) of HF. Another, Gene Oncology (GO) analysis of DECs host genes was performed to elucidate the underlying pathogenesis of HF, suggesting that binding and catalytic activity accounted for a great part of DECs. The pathways related to immune system, metabolism and signal transduction were significantly enriched. Additionally, 1052 potentially regulated miRNAs from the top 40 DECs were collected to construct a circRNA–miRNA network and found that 470 miRNAs can be regulated by multiple circRNA, while others were regulated by a single circRNA. And further comparison with the top 10 mRNAs and their targeted miRNAs in HF showed DDX3Y and UTY were regulated by the most and least circRNA, respectively. Finally, four selected randomly DECs were validated by qRT-PCR. CONCLUSION These data showed that circRNAs have species and tissue specific expression features; although circRNAs expression independent on host genes, same types of genes between DECs and DEGs worked in HF. CircRNAs could regulate miRNAs as molecular sponges. Our data would enhance understanding of the important roles of circRNAs and lay a foundation for future investigations of HF molecular functions.

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References(50)

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